Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains.
نویسندگان
چکیده
Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 x 10(6) per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells which lack cell wall and plastids, and can be considered as haploid protoplasts.
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ورودعنوان ژورنال:
- Plant physiology
دوره 85 4 شماره
صفحات -
تاریخ انتشار 1987